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iss-preprocess 0.2.1 documentation

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iss-preprocess 0.2.1 documentation

Contents:

Getting started
Crunch the data
Registration
Calling spots
Channel brightness and illumination correction
Bleedthrough matrix estimation
Calling various spots
Detecting hybridisation spots
Cell segmentation
Detection mCherry cells

API reference:

iss_preprocess package
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Channel brightness and illumination correction¶

Channel brightness¶

round1_only option¶

Illumination correction¶

Bleedthrough matrix estimation

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Channel brightness and illumination correction
- Channel brightness
  - round1_only option
- Illumination correction